3rd International Top-Down Proteomics Symposium

Top-down sequencing of intact, modified proteins by timsTOF technology with new multi-modal fragmentation capabilities

Aug 27, 2025, 8:50 AM

20m

VMP 6 / Philturm (Lecture Hall D)

Oral Presentation New Tools for Proteoform Analysis New Tools for Proteoform Analysis

Speaker

Ole Nørregaard Jensen (SDU Department of Biochemistry and Molecular Biology)

Description

We demonstrate top-down sequencing and detailed PTM characterization of intact histone proteoforms using a novel timsOMNI mass spectrometry platform. We prepared well defined acetylated and methylated proteoforms of histones H3 and H4 that were analyzed and sequenced by nanoESI interfaced to a modified timsTOF Ultra mass spectrometer equipped with the omnitrap technology (FasmaTech, Bruker Daltonics). Intact protein sequencing by MS/MS was performed by CID, ECD and combinations thereof. Data analysis was expedited by the Omniscape software (Bruker Daltonics). The timsOMNI platform generated highly informative tandem mass spectra of intact proteins enabling comprehensive sequence analysis, localization of multiple PTM sites and assessment of proteoform heterogeneity.