Name: 3rd International Top-Down Proteomics Symposium
Start: 2025-08-25T09:00:00+02:00
End: 2025-08-29T16:45:00+02:00
Location: Lecture Hall D

3rd International Top-Down Proteomics Symposium

from Monday, August 25, 2025 (9:00 AM) to Friday, August 29, 2025 (4:45 PM)

Monday, August 25, 2025

9:00 AM

9:00 AM - 5:00 PM
Room: VMP 6 / Philturm
Tuesday, August 26, 2025

8:30 AM

8:30 AM - 1:00 PM
Room: VMP 6 / Philturm

12:00 PM

12:00 PM - 12:30 PM

1:00 PM

1:00 PM - 2:30 PM
Room: VMP 6 / Philturm

2:00 PM Welcome Coffee
Welcome Coffee
2:00 PM - 3:00 PM

3:15 PM

3:15 PM - 3:30 PM
Room: VMP 6 / Philturm
3:30 PM
3:30 PM - 5:10 PM
Room: VMP 6 / Philturm
Contributions

3:30 PM New Frontiers in Proteomics - Proteoforms, Proteoform Families, and the Human Proteoform Project - Lloyd Smith (Department of Chemistry, University of Wisconsin - Madison)

3:55 PM Digitizing Proteoform Biology with Single Molecule & Single Cell Mass Spectrometry - Neil Kelleher

4:20 PM Revealing Functional Proteoforms by Native Top-Down Proteomics - Joseph Loo (UCLA)

4:45 PM Touching upon the millions of hidden treasures in the plasma proteome - Albert Heck (Utrecht University)
5:10 PM

5:10 PM - 6:10 PM
Room: VMP 6 / Philturm

6:15 PM

6:15 PM - 9:00 PM
Wednesday, August 27, 2025
8:30 AM
8:30 AM - 10:00 AM
Room: VMP 6 / Philturm
Contributions

8:30 AM Advances in hardware design and function of the new timsOmni MS platform - Dimitris Papanastasiou (Fasmatech Science & Technology)

8:50 AM Top-down sequencing of intact, modified proteins by timsTOF technology with new multi-modal fragmentation capabilities - Ole Nørregaard Jensen (SDU Department of Biochemistry and Molecular Biology)

9:10 AM Sensitive Top-down Analysis using Spray-capillary-based CE-MS approaches - Si Wu

9:30 AM Combining advanced fragmentation techniques and spectral simplification for deep proteoform interrogation - Luca Fornelli (University of Oklahoma)

9:45 AM Mass-Invariant Log-Transformed Mass Spectra Enable De Novo Sequencing and Internal Calibration of Intact Proteins - Lissa C. Anderson (National High Magnetic Field Laboratory, Florida State University)
10:00 AM Coffee Break
Coffee Break
10:00 AM - 10:30 AM
10:30 AM
10:30 AM - 12:00 PM
Room: VMP 6 / Philturm
Contributions

10:35 AM Properties, Origin, and Reproducibility of Truncated Proteoforms Across Top-Down Proteomic Studies - Philipp T. Kaulich (Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, 24105 Kiel, Germany)

10:40 AM Novel bridged hybrid monolithic columns combined with mass spectrometry for top-down proteomic analysis - Yu Liang (Dalian Institute of Chemical Physics, CAS, China)

10:45 AM Spatial Phosphoproteomic Profiling of Murine Heart Reveals Region-Specific Functions via TiO₂ Enrichment Optimized for Laser-Capture Microdissected Samples - Dan Zhao

10:50 AM FLASHApp: Interactive Data Analysis and Visualization for Top-Down Proteomics - Tom David Müller (University of Tübingen)

10:55 AM Metabolomics and proteomics reveal the inhibitory effect of Lactobacillus crispatus on cervical cancer - Lingyan Zhong

11:00 AM SEC-complex-down approaches with functional O2-affinity assay: Correlation between the higher order structure of bird hemoglobin homologues and their function. - Léa Letissier (LSMBO - CNRS (Université de Strasbourg))

11:05 AM Multi-dimensional High-Throughput Molecular Glue Screening via Gas Phase Affinity Selection Native Mass Spectrometry and Cryo-EM Analysis - Amanda Lee (Thermo Fisher Scientific)

11:10 AM Legionella effector AnkX puts the brakes on IMPDH2 filaments - Marietta Sandkamp-Kaspers (UniversitÃ¤t Hamburg)

11:15 AM Top-Down Mass Spectrometry of a Clinical Antibody Light Chain Using the Omnitrap-Orbitrap-Booster Platform - Marie Yammine (INSTITUT PASTEUR - CNRS)

11:20 AM X-ray spectroscopy meets native mass spectrometry: probing gas-phase protein complexes - Jocky Chun Kui Kung (Centre for Structural Systems Biology)

11:25 AM A prototype TIMS-FT-ICR MS instrument capable of deep characterisation of complex samples and biomolecules - Christopher Wootton (Bruker Daltonics)

11:30 AM Pin-pointing phosphorylation-dependent Pin1 binding to a cytoskeletal protein altered in Alzheimer's Disease using structural mass spectrometry - Nikolas Brooks (School of Biosciences, University of Birmingham, Birmingham, B15 2TT)

11:35 AM Enhancing Drug-Payload Localization in Antibody-Drug Conjugates with a Middle-Down Approach Utilizing Proton Transfer Charge Reduction on an Orbitrap Ascend BioPharma Tribrid Mass Spectrometer - Rachel Grady (Thermo Fisher Scientific)

11:40 AM Digital Membrane Chromatography — A New Way to Rapid Antibody Purification for Top-Down MS - Kilian Müller (UKE)
12:00 PM Lunch Seminar (Agilent Technologies)
Lunch Seminar (Agilent Technologies)
12:00 PM - 1:00 PM
Room: VMP 6 / Philturm

1:00 PM

1:00 PM - 1:45 PM
1:45 PM
1:45 PM - 2:40 PM
Room: VMP 6 / Philturm
Contributions

1:45 PM Uncovering the unique properties of circulating proteasomes: A mass spectrometry perspective - Michal Sharon (Department of Biomolecular Sciences, Weizmann Institute of Science)

2:05 PM Flying viruses – mass spectrometry meets X-rays - Charlotte Uetrecht (CSSB/DESY/University of Lübeck)

2:25 PM Filling the Structural Knowledge Gap in Protein Design via Native Mass Spectrometry - Mowei Zhou (Zhejiang University)
2:40 PM Coffee Break
Coffee Break
2:40 PM - 3:10 PM
3:10 PM
3:10 PM - 4:40 PM
Room: VMP 6 / Philturm
Contributions

3:10 PM Replacing “OR“ Logic and Mass Accuracy with “AND/OR“ Logic and Mass Resolving Power, as the Basis for Peak Assignment in Top-Down Mass Spectrometry Data. - Jeffrey Agar (Northeastern University)

3:30 PM Advancing Top- and Middle-Down Antibody Analysis Using Simulated FTMS Datasets - Yury Tsybin

3:50 PM Computational methods in top-down proteomics to address challenges in proteoform analysis - Kyowon Jeong (University of Tübingen)

4:05 PM TDAuditor assesses deconvolution quality for the Blood Proteoform Atlas - David Tabb (University Medical Center of Groningen)

4:20 PM The Implementation of Open Science Practices Can Enable A Faster Development Of Top-Down Proteomics - Juan Antonio Vizcaino (European Bioinformatics Institute (EMBL-EBI))
4:40 PM Break
Break
4:40 PM - 4:50 PM

4:50 PM

4:50 PM - 6:00 PM
Room: VMP 6 / Philturm

7:00 PM Conference Dinner
Conference Dinner
7:00 PM - 11:00 PM
Thursday, August 28, 2025
8:30 AM
8:30 AM - 9:50 AM
Room: VMP 6 / Philturm
Contributions

8:30 AM Enabling High-Throughput Proteoform Analysis via Gel-Based Sample Pre-Fractionation with PEPPI-SP3 - Nobuaki Takemori (Ehime University)

8:50 AM Characterization of proteoforms of intact proteins by CE-MS and LC-CE-MS - Christian Neusüß (Hochschule Aalen)

9:05 AM Impact of sample preparation methods on proteoform identification by top-down proteomics - Andreas Tholey

9:20 AM Proteoforms in Tissues – Approaching Their Native Composition with Nano- and Pico-Second Infrared Laser Systems - Hartmut Schlüter (Institute of Biochemistry)

9:35 AM Comparison of RP-LC with CE for histone analysis - Ansgar Poetsch (Nanchang University)
9:50 AM Coffee Break
Coffee Break
9:50 AM - 10:20 AM
10:20 AM
10:20 AM - 12:00 PM
Room: VMP 6 / Philturm
Contributions

10:20 AM Advances in Orbitrap mass spectrometry for top-down analysis - Alexander Makarov (Thermo Fisher Scientific (Bremen) GmbH)

10:40 AM Exploring Spatial Top-Down Proteomics - Ljiljana Paša-Tolić (Pacific Northwest National Laboratory, Richland, WA 99354, USA)

11:00 AM Exploring the effects of isotope depletion on proteins by native mass spectrometry and cryogenic electron microscopy - Anjusha Mathew

11:15 AM Discovering the ‘negative’ side of the proteomic landscape with top-down mass spectrometry - Cynthia Nagy (University of Oklahoma)

11:30 AM Glycoproteomics Based on Deep Learning and Data Independent Acquisition - Liang Qiao (Fudan University)

11:45 AM High resolving power meets proton transfer charge reduction: unlocking new depths in intact protein characterization - Rafael D. Melani (Thermo Fisher Scientific, San Jose)
12:00 PM Lunch Seminar (Bruker Daltonics)
Lunch Seminar (Bruker Daltonics)
12:00 PM - 1:00 PM
Room: VMP 6 / Philturm

1:00 PM

1:00 PM - 1:45 PM
1:45 PM
1:45 PM - 3:25 PM
Room: VMP 6 / Philturm
Contributions

1:45 PM Top-Down Proteomics of the Heart: Decoding Cardiac Proteoforms for Precision Medicine - Ying Ge (University of Wisconsin-Madison)

2:05 PM Dissecting the Proteoform Landscape of Prostate-Specific Antigen: Intact, Bottom-Up, and Glycomic Perspectives - Guinevere Lageveen-Kammeijer (University of Groningen)

2:25 PM Spatial Phosphoproteomic Profiling Reveals Regional Functional Heterogeneity in the Murine Heart - Ling Lin (Fudan University)

2:40 PM Spatially Resolved Proteoform Mapping in Alzheimer’s Disease Brain Tissues - Yue Sun (Dalian Institute of Chemical Physics, Chinese Academy of Sciences)

2:55 PM Top-down Proteomics Deciphers Cardiac Proteoform Landscape in Phospholamban R14del Cardiomyopathy for Precision Medicine - Holden Rogers (University of Wisconsin-Madison)

3:10 PM MALDI MS-Based Rapid Antimicrobial Susceptibility Prediction - Jia Yi (Minhang Hospital, Fudan University)
3:25 PM Coffee Break
Coffee Break
3:25 PM - 3:55 PM
3:55 PM
3:55 PM - 5:20 PM
Room: VMP 6 / Philturm
Contributions

3:55 PM Deciphering Biotherapeutic Biotransformations with Top-Down Mass Spectrometry - Julia CHAMOT-ROOKE (INSTITUT PASTEUR - CNRS)

4:15 PM Functional and structural characterization of antibodies by native-mode affinity separation-, middle-up, and top-down mass spectrometry - Manfred Wuhrer (Leiden University Medical Center)

4:35 PM AiDA Accelerates Top-Down and Middle-Down MS Data Analysis Across Multiple Antibody Variants - Francois Griaud (Novartis Pharma AG)

4:50 PM Enhanced usage of top-down data for de novo sequencing of antibodies - Kira Vyatkina (Sechenov University)

5:05 PM Mass spectrometric ITEM-FOUR analysis reveals coding single nucleotide polymorphisms in human cardiac troponin T that evade detection by sandwich ELISAs which use monoclonal antibodies M7 and M11.7 from the Elecsys Troponin T® assay - Michael Glocker
5:20 PM Poster Awards and Farewell
Poster Awards and Farewell
5:20 PM - 5:50 PM
Room: VMP 6 / Philturm
Friday, August 29, 2025

8:45 AM

8:45 AM - 4:45 PM

9:00 AM

9:00 AM - 2:00 PM
Room: VMP 6 / Philturm

Choose timezone