3rd International Top-Down Proteomics Symposium

Session

Lightning Talks

Aug 27, 2025, 10:30 AM

VMP 6 / Philturm (Lecture Hall D)

58. Properties, Origin, and Reproducibility of Truncated Proteoforms Across Top-Down Proteomic Studies

Dr Philipp T. Kaulich (Systematic Proteome Research & Bioanalytics, Institute for Experimental Medicine, Christian-Albrechts-Universität zu Kiel, 24105 Kiel, Germany)

8/27/25, 10:35 AM

Lightning Talk

Oral Presentation

Top-down proteomics (TDP) offers a powerful approach to identifying intact proteoforms, inherently providing information about protein modifications. Truncated proteoforms are among the most frequently observed modifications in TDP studies. Here, we selected fifty datasets, including over 140,000 proteoforms, published over the past decade, spanning various organisms, sample preparation...

3. Novel bridged hybrid monolithic columns combined with mass spectrometry for top-down proteomic analysis

Dr Yu Liang (Dalian Institute of Chemical Physics, CAS, China)

8/27/25, 10:40 AM

Lightning Talk

Oral Presentation

Top-down proteomics could offer comprehensive investigations of proteoforms. However, it is greatly challenged by the co-elution of intact proteins which results in overlapped mass spectra. Hence, highly effective protein separation to reduce the co-elution of intact proteins from complex samples is of vital importance.
The ethane-bridged hybrid monolithic column with homogeneous macropores...

23. Spatial Phosphoproteomic Profiling of Murine Heart Reveals Region-Specific Functions via TiO₂ Enrichment Optimized for Laser-Capture Microdissected Samples

Dan Zhao

8/27/25, 10:45 AM

Lightning Talk

Oral Presentation

Phosphorylation-mediated signaling dynamics across spatially distinct cardiac regions remain poorly understood due to limited technical capacity for deep and sensitive analysis of minute samples. Here, we present an optimized TiO₂-based micropipette tip method for deep phosphoproteomics, achieving high sensitivity (12,117 class I phosphosites from only 10 µg HeLa peptides) and reproducibility....

24. FLASHApp: Interactive Data Analysis and Visualization for Top-Down Proteomics

Tom David Müller (University of Tübingen)

8/27/25, 10:50 AM

Lightning Talk

Oral Presentation

Introduction
Top-down proteomics (TDP) is increasingly being applied in proteoform-resolved biomedical and clinical research. The complexity of TDP data demands flexible visualization tools integrated with analysis workflows to streamline interpretation and validation. Existing tools often lack adaptability and interactivity, requiring complimentary manual analysis to generate...

32. Metabolomics and proteomics reveal the inhibitory effect of Lactobacillus crispatus on cervical cancer

Lingyan Zhong

8/27/25, 10:55 AM

Lightning Talk

Oral Presentation

Cervical cancer remains a significant global health issue due to its high morbidity and mortality rates. Recently, Lactobacillus crispatus has been recognized for its crucial role in maintaining cervical health. While some studies have explored the use of L. crispatus to mitigate cervical cancer, the underlying mechanisms remain largely unknown. In this study, we employed non-targeted...

34. SEC-complex-down approaches with functional O2-affinity assay: Correlation between the higher order structure of bird hemoglobin homologues and their function.

Léa Letissier (LSMBO - CNRS (Université de Strasbourg))

8/27/25, 11:00 AM

Lightning Talk

Oral Presentation

Hemoglobin is a tetrameric protein responsible for the blood oxygen transport. Any abnormalities in hemoglobin structure can lead to serious health outcomes. Although very well characterized in humans, it has been scarcely studied in the case of birds. Some studies have been carried out at the globin level without providing further evidences about the native structure of the complex. In this...

38. Multi-dimensional High-Throughput Molecular Glue Screening via Gas Phase Affinity Selection Native Mass Spectrometry and Cryo-EM Analysis

Amanda Lee (Thermo Fisher Scientific)

8/27/25, 11:05 AM

Lightning Talk

Oral Presentation

Targeted protein degradation of undruggable proteins is transformative in drug discovery. Molecular glues (MGs) enhance weak interactions between targets and E3 ligase. Native mass spectrometry (nMS) identifies E3-MG-target complexes directly, but manual sample preparation limits throughput.

This study demonstrates high-throughput MG screening using nMS for WEE1 binding to CRBN-DDB1,...

39. Legionella effector AnkX puts the brakes on IMPDH2 filaments

Marietta Sandkamp-Kaspers (Universität Hamburg)

8/27/25, 11:10 AM

Lightning Talk

Oral Presentation

Bacterial pathogens often utilize post-translational modifications to manipulate host cells. Legionella pneumophila, the causative agent of Legionnaires' disease, secretes the enzyme AnkX, which is known to use cytidine diphosphate-choline to post-translationally modify the human small G-protein Rab1 with a phosphocholine moiety. Later during the infection, the Legionella enzyme Lem3 acts as a...

42. Top-Down Mass Spectrometry of a Clinical Antibody Light Chain Using the Omnitrap-Orbitrap-Booster Platform

Marie Yammine (INSTITUT PASTEUR - CNRS)

8/27/25, 11:15 AM

Lightning Talk

Oral Presentation

The Omnitrap-Orbitrap-Booster (OOB) mass spectrometry (MS) platform with enhanced high-resolution, fragmentation and data acquisition capabilities, was developed to advance top-down (TD) MS analysis of proteins. It integrates a multimodal tandem mass spectrometry (MS/MS) ion trap system (OmnitrapTM) offering a wide range of fragmentation methods, a high-resolution Orbitrap Fourier transform...

43. X-ray spectroscopy meets native mass spectrometry: probing gas-phase protein complexes

Dr Jocky Chun Kui Kung (Centre for Structural Systems Biology)

8/27/25, 11:20 AM

Lightning Talk

Oral Presentation

X-ray activation and dissociation of proteins and their non-covalent assemblies may elucidate structural and functional details complementary to established top-down mass-spectrometry techniques. This is attributed to the rapid, site-specific ionization of atoms within biomolecules. Our research group conducted proof-of-concept experiments exploring X-ray activation of samples with masses...

55. A prototype TIMS-FT-ICR MS instrument capable of deep characterisation of complex samples and biomolecules

Christopher Wootton (Bruker Daltonics)

8/27/25, 11:25 AM

Lightning Talk

Oral Presentation

Trapped ion mobility spectrometry (TIMS) spatially separates ions when suspended in a high-pressure region between a retarding electric field gradient and constant gas flow from the atmospheric pressure inlet of the mass spectrometer. TIMS allows high resolution separation of ions/isomers/conformers with varying duty cycles in a relatively small device (<10cm). TIMS is also particularly well...

57. Pin-pointing phosphorylation-dependent Pin1 binding to a cytoskeletal protein altered in Alzheimer's Disease using structural mass spectrometry

Mr Nikolas Brooks (School of Biosciences, University of Birmingham, Birmingham, B15 2TT)

8/27/25, 11:30 AM

Lightning Talk

Oral Presentation

Abnormal protein phosphorylation is a fundamental trigger in the pathogenesis of Alzheimer’s Disease, leading to the formation of neurofibrillary tangles. Thus, molecular determination of the critical factors in controlling phosphorylation is in high demand. Pin1, a cis-trans prolyl isomerase has recently been implicated in Alzheimer’s Disease progression. Moreover, Pin1 specifically targets...

63. Enhancing Drug-Payload Localization in Antibody-Drug Conjugates with a Middle-Down Approach Utilizing Proton Transfer Charge Reduction on an Orbitrap Ascend BioPharma Tribrid Mass Spectrometer

Rachel Grady (Thermo Fisher Scientific)

8/27/25, 11:35 AM

Lightning Talk

Oral Presentation

Monoclonal antibodies (mAbs) have revolutionized biotherapeutics, offering effective treatments for various diseases. Antibody-drug conjugates (ADCs) combine mAbs’ specificity with potent cytotoxic drugs linked via Lys- or Cys-conjugation, targeting malignant cells. However, heterogeneity in payload attachment challenges ADCs' safety and efficacy. We employed a middle-down (MD) mass...

95. Digital Membrane Chromatography — A New Way to Rapid Antibody Purification for Top-Down MS

Kilian Müller (University Medical Center Hamburg-Eppendorf)

8/27/25, 11:40 AM

Lightning Talk

Oral Presentation

Antibody purification is a critical step in manufacturing and analytical workflows, yet conventional Protein A affinity chromatography often requires acidic elution, risking structural alterations and necessitating additional processing for mass spectrometry (MS). We present a Protein A-functionalized regenerated cellulose membrane adsorber with conductive gold-coated layers, enabling...