Speaker
Description
To analyze tissue molecules accurately, they must be first solubilized. Conventional sampling and homogenization methods disrupt cell compartments, releasing enzymes that can alter proteoforms through proteolysis and modifications of post-translational modifications (PTMs), thereby changing their original composition.
Using nano- or pico-second infrared laser systems (NIRL or PIRL) for tissue sampling minimizes this issue significantly. Their ultrafast sampling and homogenization processes prevent enzymatic activity from altering proteoforms, preserving their native state. Moreover, the gentle, rapid approach reduces fragmentation of proteoforms during sample preparation.
The current NIRL and PIRL systems achieve a spatial resolution of approximately 20 x 20 x 20 µm voxels, approaching single-cell resolution. In summary, NIRL and PIRL-based sampling and homogenization techniques enable a more accurate representation of proteoforms in tissues, bringing analysis closer to their original biological state.
| User consent | yes |
|---|
