Enzymes are powerful molecules for highly efficient and sustainable chemical synthesis. However, natural enzymes often have limitations and require optimization for large-scale industrial applications. Propelled by artificial intelligence, computational advances such as AlphaFold opened new venues for structure-based enzyme design. However, experimental validation still largely relies on high...
Proteasomes are well-known mediators of intracellular proteostasis, yet their role in the extracellular space remains largely unexplored. Our recent study investigates the molecular architecture and functional specialization of freely circulating 20S proteasomes (c20S) in the bloodstream. Leveraging a CRISPR-engineered transgenic mouse model, we purified c20S complexes and applied a...
