Proteoforms - encompassing the diverse protein products arising from alternative splice isoforms, genetic variations, and posttranslational modifications (PTMs) originating from a single gene - are fundamental drivers in biology. Top-down mass spectrometry (MS)-based proteomics (TDP), analyzing whole proteins without digestion, offers a comprehensive perspective of proteoforms, which is...
Early detection of prostate cancer (PCa) using prostate-specific antigen (PSA) serum levels suffers from poor specificity and sensitivity, frequently causing unnecessary treatment or missed diagnoses. To overcome this, detailed characterization of PSA proteoforms, especially regarding glycosylation, is essential. Here, we employed an integrated analytical strategy using capillary...
Phosphorylation mediated signaling is fundamental to cardiac function. However, the dynamic signaling patterns across different spatial regions of the heart have been inadequately explored. This is mainly because of the technical difficulties in analyzing tiny tissue samples with the required depth and sensitivity. To tackle these challenges, we developed an optimized TiO₂ based micropipette...
Understanding spatial heterogeneity of proteoforms has great potential to unravel physiological and disease mechanisms. However, conventional proteomics often lacks spatial information or focuses on the protein-coding gene level, while existing methods for spatial proteoform analysis suffer from low throughput or limited coverage. These gaps hinder the exploration of spatially resolved...
Phospholamban (PLN) is a transmembrane protein that regulates cardiomyocyte calcium handling and contraction. PLN function is dynamically regulated by post-translational modifications (PTMs), most notably through phosphorylation. A pathogenic deletion of arginine-14 (PLN-R14del), is associated with dilated cardiomyopathy (DCM), and defined by a high mortality rate with minimal treatment...
Bacterial infections are among the diseases with high morbidity and mortality rates worldwide, posing a significant threat to global public health. There is an urgent need to develop precise and rapid diagnostic methods for bacterial infections to enable personalized medication and treatment for infected patients, promptly save lives, and reduce the spread of antimicrobial resistance....
Introduction:
AMP-activated kinase (AMPK) complex is the regulatory hub of cell energy metabolism, linked to heart disease and longevity, making it an attractive drug target. AMPK activity involves sophisticated subunit isoforms, conformational changes, and post-translational modifications. Phosphorylation controls AMPK through upstream kinases, generating diverse proteoforms whose extent and...
