Proteins are the primary effectors of function in biology, and thus complete knowledge of their structure and behavior is needed to decipher function. However the richness of protein structure and function goes far beyond the linear amino acid sequence dictated by the genetic code. Multigene families, alternative splicing, coding polymorphisms, and post-translational modifications, work...
Since the completion of the Human Genome Project, much has been made of the need to bridge the gap from genes and traits. As a key nexus for the many interacting ‘-omes’ (genome, transcriptome, proteome, metabolome, etc.), the proteome should offer a tight link between genotype and phenotype. Proteoforms, or all of the precise molecular forms of a protein, capture all sources of variability in...
Native mass spectrometry (nMS) measures proteins and complexes that are functionally relevant to biology. Top-down proteomics (TDP) reveals identification, sequence, and proteoform information. The combination of these platforms, native top-down proteomics (nTDP), could be ideal for understanding how proteins interact with other proteins (and ligands and cofactors), identifying unknown...
I will describe how innovative techniques in mass spectrometry provide unique novel insights into our humoral immune response. In our body we produce every day huge amounts of antibodies, of which many end up in circulation. Humans can make about trillions of distinct antibody clones, all exhibiting a different sequence, recognizing distinct antigens. We recently developed new LC-MS based...
