Introduction
Protein-centric proteomics is an emerging mass spectrometric field where to answer a biological question related to cells or tissues, one analyzes proteins directly, without relying on their digestion into smaller peptides. The unique ability of protein-centric proteomics to disentangle isoforms and proteoforms is directly beneficial to the complex functional analysis usually...
Methionine oxidation is one of the most frequently observed post-translational modifications of monoclonal antibody (mAb) products during their development and production that can affect the drug’s safety and efficacy. Such modifications are typically assessed using bottom-up peptide mapping techniques. However, this methodology is often tedious and prone to the generation of artefacts through...
Top-down mass spectrometry (TDMS) elucidates the molecular weight, characterizes proteoforms, and, in its native form, can even inform binding stoichiometry and higher-order structure of proteins and their complexes. The information gained is, however, largely dependent on the protein system in question and the dissociation technique used. Therefore, there is a great need for more efficient...
Protein–protein interactions (PPIs) govern countless biological processes and range from stable, permanent complexes to transient, short-lived ones. Among the latter, short linear motifs (SLiMs) play a critical role by mediating low-affinity, transient interactions. These are essential for cellular functions like directing protein localization through targeting signals. However, due to their...
Dynamically altering biomolecules are the true actors in studies on health and disease rather than genotypes or phenotypes that refer to a certain risk or an end-point observation. Unfortunately, clinically effective disease-specific tests that support diagnoses at an early and curable stage are still lacking for a wide variety of diseases. Proteoform-resolved data provides a layer of...
Nucleic acids (DNA, RNA, artificial analogues such as TNA) can form remarkable three-dimensional structures, which play key roles in biology and nanobiotechnology. In the field of DNA nanotechnology, static or dynamic nucleic acid assemblies have applications in biotechnology, nanomedicine, nanophotonics and nanoelectronics. Determining the size, shape and assembly route of oligomeric nucleic...
Ovarian tissue cryopreservation (OTC) is utilized by clinics to preserve oocytes and immature eggs for pediatric patients who are at increased risk of becoming infertile due to a diagnosis or treatment. Late-stage oocytes, that are not yet mature, are often released during OTC. We are interested in identifying proteoforms that may improve in vitro maturation of oocytes into eggs for these...
Monoclonal antibodies (mAbs) are biopharmaceuticals widely used in treatment of various diseases. Due to their intrinsic complexity and post-translational modifications, therapeutic mAbs are highly heterogeneous molecules requiring extensive quality control. Quantitative analysis typically relies on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and the gold-standard...
