Speaker
Kilian Müller
(University Medical Center Hamburg-Eppendorf)
Description
Antibody purification is a critical step in manufacturing and analytical workflows, yet conventional Protein A affinity chromatography often requires acidic elution, risking structural alterations and necessitating additional processing for mass spectrometry (MS). We present a Protein A-functionalized regenerated cellulose membrane adsorber with conductive gold-coated layers, enabling voltage-triggered elution—termed Digital Membrane Chromatography (DMC). Using Trastuzumab as a model, DMC achieved efficient antibody capture and mild, MS-compatible elution without desalting or buffer exchange. This approach preserves structural integrity, supports analysis from complex matrices, and reduces processing time compared to conventional methods.
Author
Kilian Müller
(University Medical Center Hamburg-Eppendorf)
Co-authors
Julia Rößler
(i3 Membrane)
Anna Klassen
(i3 Membrane)
Hannes Passuth
(i3 Membrane)
Florian Schmitt
(i3 Membrane)
Bente Siebels
(University Medical Center Hamburg-Eppendorf)
Hartmut Schlüter
(University Medical Center Hamburg-Eppendorf)
Maria Riedner
(University of Hamburg)
